Principle Microbiology

GFP-Photo reaction:

· The GFP absorps mainly at 396 nm, followed by loss of proton in excited state to form phenolate.

· The main emmision occurs at 508 nm. Small amount of GFP absorps at 475 nm and emits green light at 508 nm.

· The GFP as well as green fluoroscent gene can be modified. The gene can be modified by mutation.

· The fluoroscent may be partially or completely lost. The mutation gives some misfolding of protein that generally fails to produce fluoroscence, but sometimes also produce higher emission.